Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.

نویسندگان

  • K Tang
  • D Fu
  • S Kötter
  • R J Cotter
  • C R Cantor
  • H Köster
چکیده

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, ammonium citrate matrix. The strategy has several applications, such as fast DNA sequence analysis and DNA diagnostics.

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عنوان ژورنال:
  • Nucleic acids research

دوره 23 16  شماره 

صفحات  -

تاریخ انتشار 1995